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rabbit polyclonal anti nf κb p65 antibody  (Bioss)


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    Bioss rabbit polyclonal anti nf κb p65 antibody
    Rabbit Polyclonal Anti Nf κb P65 Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+nf+%CE%BAb+p65+antibody/pm42105711-98-12-18?v=Bioss
    Average 95 stars, based on 199 article reviews
    rabbit polyclonal anti nf κb p65 antibody - by Bioz Stars, 2026-08
    95/100 stars

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    RNA-seq of HMEC-1. (A) Volcano plots of DEGs (n = 5). (B) KEGG enrichment bubble plots of DEGs (n = 5). (C) DEGs related to vascular endothelial injury. (D) The relative mRNA expression levels of vascular endothelial injury-related genes verified by RT-qPCR (n = 3). (E) The relative mRNA expression levels of TLR4, TRIF and <t>p65</t> were detected by RT-qPCR (n = 3). (F) The expression protein levels of TLR4, TRIF and p-p65/p65 were detected by Western blot (n = 3). Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01. The screening criteria for DEGs were defined as FC ≥ 2 or ≤0.5, FDR < 0.05 and p -value <0.05.
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    RNA-seq of HMEC-1. (A) Volcano plots of DEGs (n = 5). (B) KEGG enrichment bubble plots of DEGs (n = 5). (C) DEGs related to vascular endothelial injury. (D) The relative mRNA expression levels of vascular endothelial injury-related genes verified by RT-qPCR (n = 3). (E) The relative mRNA expression levels of TLR4, TRIF and <t>p65</t> were detected by RT-qPCR (n = 3). (F) The expression protein levels of TLR4, TRIF and p-p65/p65 were detected by Western blot (n = 3). Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01. The screening criteria for DEGs were defined as FC ≥ 2 or ≤0.5, FDR < 0.05 and p -value <0.05.
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    RNA-seq of HMEC-1. (A) Volcano plots of DEGs (n = 5). (B) KEGG enrichment bubble plots of DEGs (n = 5). (C) DEGs related to vascular endothelial injury. (D) The relative mRNA expression levels of vascular endothelial injury-related genes verified by RT-qPCR (n = 3). (E) The relative mRNA expression levels of TLR4, TRIF and <t>p65</t> were detected by RT-qPCR (n = 3). (F) The expression protein levels of TLR4, TRIF and p-p65/p65 were detected by Western blot (n = 3). Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01. The screening criteria for DEGs were defined as FC ≥ 2 or ≤0.5, FDR < 0.05 and p -value <0.05.
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    Image Search Results


    RNA-seq of HMEC-1. (A) Volcano plots of DEGs (n = 5). (B) KEGG enrichment bubble plots of DEGs (n = 5). (C) DEGs related to vascular endothelial injury. (D) The relative mRNA expression levels of vascular endothelial injury-related genes verified by RT-qPCR (n = 3). (E) The relative mRNA expression levels of TLR4, TRIF and p65 were detected by RT-qPCR (n = 3). (F) The expression protein levels of TLR4, TRIF and p-p65/p65 were detected by Western blot (n = 3). Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01. The screening criteria for DEGs were defined as FC ≥ 2 or ≤0.5, FDR < 0.05 and p -value <0.05.

    Journal: Frontiers in Pharmacology

    Article Title: Naoxintong capsule decreases circulating exosomes of miR-382-5p to protect LPS-induced vascular endothelial cell injury by targeting STC1 in vitro

    doi: 10.3389/fphar.2026.1655883

    Figure Lengend Snippet: RNA-seq of HMEC-1. (A) Volcano plots of DEGs (n = 5). (B) KEGG enrichment bubble plots of DEGs (n = 5). (C) DEGs related to vascular endothelial injury. (D) The relative mRNA expression levels of vascular endothelial injury-related genes verified by RT-qPCR (n = 3). (E) The relative mRNA expression levels of TLR4, TRIF and p65 were detected by RT-qPCR (n = 3). (F) The expression protein levels of TLR4, TRIF and p-p65/p65 were detected by Western blot (n = 3). Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01. The screening criteria for DEGs were defined as FC ≥ 2 or ≤0.5, FDR < 0.05 and p -value <0.05.

    Article Snippet: After transfer, the membranes were blocked with sealing liquid and incubated for 2 h. Following blocking, membranes were incubated with primary antibodies against β-actin (dilution 1:1,000, ab8227, Abcam), ALIX (dilution 1:1,000, ab275377, Abcam), TSG101 (dilution 1:1,000, ab125011, Abcam), CD9 (dilution 1:1,000, ab236630, Abcam), Bax (dilution 1:1,000, ab32503, Abcam), Bcl-2 (dilution 1:1,000, ab32124, Abcam), Cleaved caspase 3 (dilution 1:500, ab32042, Abcam), TLR4 (dilution 1:1,000, TA382568, OriGene), TRIF (dilution 1:1,000, TA382523, OriGene), NF-κB p65 (dilution 1:1,000, TA385159, OriGene), Phospho-NF-κB p-p65 (dilution 1:1,000, TA380848, OriGene), STC1 (dilution 1:5000, ab229477, Abcam) at 4 °C overnight.

    Techniques: RNA Sequencing, Expressing, Quantitative RT-PCR, Western Blot